A man has been arrested on suspicion of murder following the attack at the TUI branch in Southport on Saturday.
Officers were called to Chapel Street at 1.25pm and the woman was taken to hospital, where she later died from her injuries.
A 30-year-old man from the Southport area was detained over the incident, which is believed to be domestic related.
The woman’s next of kin have been informed, police added.
A police cordon has been set up around the store and forensic officers are carrying out their investigations.
Southport: forensics officer has now entered Tui. He’s working inside the shop now pic.twitter.com/C3XfMamqti
A spokeswoman for Merseyside Police said: “We can confirm a murder investigation has been launched following an incident in Southport today.
“Detectives are appealing for anyone with information to contact Merseyside Police on 101 quoting log 470 of January 13 or the Crimestoppers line anonymously on 0800 555 111.”
A TUI spokeswoman said: “We regret to confirm that a female member of staff at our Southport TUI retail store tragically died in an incident today.
“We send our deepest sympathies to the friends and family of the staff member involved.
“We’re doing everything possible to assist the local police with their investigation and support our customers and staff at this difficult time.”
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